![]() Visit the Gate Cut-off page for information on setting the X 2 threshold.Ĭlick the clipboard to copy this table to clipboard. button at the bottom of the window to set the number of bins.Ī gate based on the differences between the two samples can be created by clicking Create. The number of bins can be set according to the number of events collected. All of the samples can be designated controls by clicking the Set All button.Ĭheck the boxes on the left to choose the Parameters to Compare. Click the Set button (under Select controls) to designate these samples as controls (they become red). Select the control samples in the list by clicking (they become shaded). Note that this platform does not associate with a particular population.ĭrag two or more samples (or subsets of the same sample) to the right side of the platform into the Populations and Statistics box. Start by selecting the Multi-sample Comparison Platform from the Platforms menu. Caution is warranted since reduction of the number of samples entered as controls can lead to sampling bias. Outliers can be removed from the control set. One can concatenate all the control samples and compute the distances of each control sample to the average of all of them. This process mitigates the potential artifact introduces by selection of a sample as control that is actually significantly differentĭetermining the samples to be concatenated is best approached by an iterative process. ![]() In such a case, the Multi-sample Comparison platformĬan concatenate all of the control samples in order to use the average of all the control samples for comparison. In FlowJo, the Multi-sample Comparison platform is used to compare single or multiple parameters from a test sample to a composite of more than one control sample.įor example, in comparing multiple samples against each other, it is sometimes not possible (or meaningful) to assign a single sample as the control, against which all others are to be compared.
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